Journal: PLOS Pathogens
Article Title: The efficiency of rotavirus A spread to extraintestinal tissues is not determined by the levels of its replication in the gut
doi: 10.1371/journal.ppat.1013723
Figure Lengend Snippet: Representative fluorescence microscopy images (A) show DAPI-stained nuclei (blue), highlighting tissue architecture. Sialylated glycans were detected using FITC-labeled Sambucus nigra agglutinin (SNA) and Maackia amurensis I lectin (MAL I) , which are specific to α2,6- and α2,3-linked sialic acids, respectively. Non-sialylated glycans were detected using FITC-labeled peanut agglutinin (PNA) and Griffonia simplicifolia lectin I (GSL I) , which are specific to galactose and α-galactose, respectively (green).Histo-blood group antigens A (HBGA-A) and H (HBGA-H) were detected using specific antibodies, followed by FITC-labeled goat anti-mouse IgG/A/M (H + L) secondary antibody (1:750; Bio-Rad).The expression levels of glycans were analyzed (B) by calculating the corrected total cell fluorescence (CTCF) .
Article Snippet: α2,3 sialic acid , Maackia amurensis I lectin (MAL I) , 5μg/ml , Vector Laboratories.
Techniques: Fluorescence, Microscopy, Staining, Labeling, Expressing